TGF alpha antibody orb248561 from Biorbyt

Description

TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody

Product Specs
ItemTGF alpha antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb248561
Quantity20 µg, 100 µg
ApplicationsELISA, IHC-P
Conjugate/TagUnconjugated
PurityProtein G Affinity Chromatography
ReactivityHuman, Mouse, Rat
TargetTGF alpha
IsotypeMouse IgG1, kappa
CloneMF9
HostMouse
ImmunogenA 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody
NCBI Full Gene Nametransforming growth factor alpha
NCBI Gene AliasesTFGA
ClonalityMonoclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide)

Specifications/Features

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Application Dilutions: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

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